• Document: ABSTRAK. PENGARUH EKSTRAK KULIT LEMON (Citrus limon Linn.) TERHADAP PERTUMBUHAN Candida albicans PADA LANDASAN GIGI TIRUAN RESIN AKRILIK HEAT CURED
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ABSTRAK PENGARUH EKSTRAK KULIT LEMON (Citrus limon Linn.) TERHADAP PERTUMBUHAN Candida albicans PADA LANDASAN GIGI TIRUAN RESIN AKRILIK HEAT CURED Maria Clara Angelina, 2014. Pembimbing I : Dahlia Sutanto, drg., Sp.Pros Pembimbing II : Triswaty Winata, dr., M.Kes Latar Belakang Penggunaan gigi tiruan lepasan merupakan salah satu penyebab terjadinya perubahan mikroflora rongga mulut pada individu tertentu, perubahan lingkungan tersebut dapat menjadi penyebab denture stomatitis. Infeksi Candida albicans merupakan penyebab utama denture stomatitis pada 60% pengguna gigi tiruan lepasan. Pencegahan dapat dilakukan dengan menjaga kebersihan gigi tiruan menggunakan denture cleanser. Tujuan Penelitian Untuk mengetahui pengaruh ekstrak kulit lemon (Citrus limon Linn.) dalam menghambat pertumbuhan Candida albicans pada landasan gigi tiruan resin akrilik heat cured. Metode Penelitian Penelitian ini menggunakan metode eksperimental laboratorium. Sampel penelitian adalah 25 plat akrilik berukuran 10x10x2 mm. Plat yang telah dikontaminasi dengan Candida albicans direndam dalam ekstrak kulit lemon konsentrasi 5%, 10%, 20%, 40% dan akuades selama 10 menit. Koloni Candida albicans dihitung setelah media diinkubasi selama 48 jam pada suhu 37oC. Hasil penelitian dianalisa dengan uji One-Way ANOVA dan Tukey. Hasil Terdapat perbedaan yang signifikan antara ekstrak kulit lemon dengan konsentrasi yang berbeda terhadap pertumbuhan Candida albicans (p≤0,05). Jumlah koloni Candida albicans dalam ekstrak kulit lemon konsentrasi 20% dan 40% lebih sedikit dibandingkan dengan semua kelompok perlakuan. Kesimpulan Ekstrak kulit lemon (Citrus limon Linn.) dapat menghambat pertumbuhan Candida albicans pada landasan gigi tiruan resin akrilik heat cured. Perendaman dalam ekstrak kulit lemon konsentrasi 20% dan 40% paling efektif menghambat pertumbuhan Candida albicans. Kata kunci : Denture stomatitis, Candida albicans, ekstrak kulit lemon, landasan gigi tiruan resin akrilik heat cured. iv ABSTRACT THE EFFECT OF LEMON (Citrus limon Linn.) PEEL EXTRACT TO THE GROWTH OF Candida albicans ON HEAT CURED ACRYLIC RESIN DENTURE BASE Maria Clara Angelina, 2014. 1st Supervisor : Dahlia Sutanto, drg., Sp.Pros 2st Supervisor : Triswaty Winata, dr., M.Kes Background Wearing removable dental prosthesis causes an conversion the oral microflora for certain individuals, this new environment is responsible for the development of denture stomatitis. Candida albicans infection is a major cause of denture stomatitis in 60% removable denture users. The prevention of denture stomatitis is by keeping clean the denture using denture cleanser. Objective Purpose of this research is to determine the effect of lemon (Citrus limon Linn.) peel extract to inhibit the growth of Candida albicans on heat cured acrylic resin denture base. Method This study applies a laboratory experimental method. The samples were 25 acrylic plates with 10x10x2 mm in size. Plate that had been contaminated with Candida albicans immersed in lemon peel extract 5%, 10%, 20%, 40% concentrations and distilled water for 10 minutes. Candida albicans colonies were counted after the media incubated for 48 hours at 37oC. The data were analyzed by the One-Way ANOVA and Tukey test. Result There were significant differences among different concentration of lemon peel extract to inhibit the growth of Candida albicans (p≤0,05). The number of Candida albicans colonies in lemon peel extract 20% and 40% concentration were lower compare to all treatment groups. Conclusion Lemon (Citrus limon Linn.) peel extract inhibits the growth of Candida albicans on heat cured acrylic resin denture base. The immersion in lemon peel extract 20% and 40% concentration is the most effective inhibiting the growth of Candida albicans. Keywords : Denture stomatitis, Candida albicans, lemon peel extract, heat cured acrylic resin denture base. v viii DAFTAR ISI HALAMAN JUDUL ........................................................................................ i LEMBAR PERSETUJUAN PEMBIMBING ............................................... ii SURAT PERNYATAAN ................................................................................ iii ABSTRAK ........................................................................................................ iv ABSTRACT ....................................................................................................... v PRAKATA ........................................................................................................ vi DAFTAR ISI .........................................................................

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