• Document: Mathematics Exercises in Biotechnology
• Size: 2.41 MB
• Status: Successfully converted

Some snippets from your converted document:

Mathematics Exercises in Biotechnology Edited by Mary Ann Hovis Robert L. Kimball John C. Peterson February 11, 2005 NSF Award #: DUE 0003065 i Contents Preface vi Cross-Reference Table, by Chapter, of Mathematics and Biotechnology Areas viii I Concentration/Dilution 1 1 Concentration of Egg White Lysozyme 2 2 Restriction Digestion of DNA 4 3 Using a Standard Graph to Determine the Concentration of an Unknown Solu- tion 6 4 Determining the Number of Bacteria in a Culture 8 5 Serial Dilutions 10 6 Dilutions of Stock Liquid Solutions 12 7 Defining and Distinguishing Between Concentration and Amount of Solute 14 8 Absorbance and Transmittance in a Spectrophotometer 17 9 Creatine Kinase and Tissue Injury 19 10 PCR Laboratory Using Custom Primers 21 11 Cytotoxicity Testing of Anti-Cancer Drugs 23 12 Soft agar cloning 25 Work on this project was partially funded by the National Science Foundation. NSF Award #: DUE 00 03065 ii 13 Cell Culture Medium 27 II Solution Preparation 29 14 Primer Preparation 30 15 Buffer Preparation 32 16 Percent Solution Conversion 34 17 Preparation of % solutions 36 18 Calculating How Much Vitamin to Add to Nutrient Broth in a Company Work- place 38 19 Gel electrophoresis reagent 40 20 One Hundred Percent Correct Each Time: Preparing Solutions for the Biotech- nology Industry 42 III Serial Dilution 45 21 Saving the Cell Culture 46 22 Test for Endotoxin: A Serial Dilution Problem 48 IV Calibration 51 23 Monthly Check on Pipetter Accuracy and Precision 52 V Molarity 54 24 Salinity Difference 55 25 Reagent Preparation Using Weight to Volume 57 26 Purification of Lactate Dehydrogenase (LDH) 59 Work on this project was partially funded by the National Science Foundation. NSF Award #: DUE 00 03065 iii 27 Preparation of laboratory solutions 62 28 Do Conversions in Your Head (but document the process if working in industry) 64 VI Radioactive Decay 66 29 Hot Stuff ! 67 VII Cell Growth 69 30 Carrot Culture 70 31 Substrate Conversion

Recently converted files (publicly available):